BACKGROUND : Prelabour rupture of membrane is a major cause of preterm birth. This is also associated with maternal and perinatal infections, morbity and mortality. Diagnosis in obvious cases is simple and straightforward. However, there maybe difficulty in making diagnosis in equivocal cases. Biochemical markers such as creatinine and beta human chorionic gonadotrophin have been invaluable in making diagnosis in such cases. AIM :The overall aim is to compare the reliability of vaginal creatinine with quantitative beta human chorionic gonadotrophin in the diagnosis of prelabour rupture of membrane. METHODS/MATERIALS: 200 parturients who met the inclusion criteria were divided into three groups. Group I were women with positive vaginal leakage and positive fluid leakage from the cervix on sterile speculum examination. Group II were women with positive vaginal leakage and negative fluid leakage from the cervix on sterile speculum examination or negative nitrazine test. Group III consisted of normal pregnant women without any complaints. . All participants were sampled for cervico-vaginal fluid creatinine and beta human chorionic gonadotrophin by speculum examination. One-way ANOVA, chi-square, correlation coefficient and receiver operator characteristics (ROC) curve analysis were used.