Obstructed labour is a major cause of maternal and neonatal morbidity and mortality with the number of maternal deaths varying between 4% and7% of all maternal deaths amounting to a maternal mortality rate as high as 410/100,000 live births7 . Available literature suggest that in many countries, maternal mortality due to obstructed labour is almost as prevalent today as it was 30 years ago7 , largely due to ruptured uterus or puerperal sepsis. This study was done to determine the pattern of pathologic colonization of the genital tract of women with obstructed labour who presented at Federal Medical Centre Owerri, to elucidate the specific organisms colonizing the genital tract of these women and to determine the antibiotic sensitivity of the organisms cultured. It is a prospective descriptive study. This study was conducted at Federal Medical Centre, Owerri Imo state from November 2014 to July 2016. Seventy patients (70) were recruited for this study in the labour ward. Patients who fulfilled the inclusion criteria were enrolled consecutively until the sample size was achieved. General and obstetric examination and vaginal examination was performed to confirm the diagnosis of obstructed labour. Having confirmed the diagnosis, after obtaining an informed consent, the participant’s biodata and other relevant information were obtained by the use of a questionnaire administered by the researcher. High vaginal swab sample was taken from each participant under aseptic conditions prior to antibiotic administration. Cultivation of samples was in duplicate for aerobes and anaerobes. The swab stick was transported to the laboratory using Amies transport medium which were plated and cultured. Antimicrobial sensitivity testing was carried out.