Pain in neonates has been unrecognised, ignored or under-treated in most neonatal service delivery systems and may have short- and long-term adverse consequences. Procedures associated with pain in neonates are still commonly carried out without pain relievers. The use of pharmacological agents as pain reliever in neonates raises concern about their potential adverse effects. The need then arises to study an effective non-pharmacological pain control agent with minimal or no side effects in neonates. This present study was, therefore, undertaken to determine the efficacy of direct breastfeeding in pain control during venepuncture among term neonates using the premature infant pain profile (PIPP). This study was a randomised controlled trial with cross-over design where each neonate served as his or her own control. It was carried out at the postnatal ward and Special Care Baby Unit (SCBU) of Ladoke Akintola University of Technology Teaching Hospital, Osogbo. Selected neonates that had direct breastfeeding first with venepuncture, were breastfed two minutes before, during and after venepuncture while the others had venepuncture when not breastfeeding . The sequence of the options was interchanged for second venepuncture after an interval of 24 hours with the same baby serving as cross-over controls. Pain was assessed using the PIPP to score different parameters such as gestational age, behavioural state, maximum heart rate, minimum oxygen saturation and facial actions (video captured) such as brow bulge, eyes squeeze and nasolabial fold. Additional information such as neonate’s biodata, vital signs, interval between venepuncture and last feed, site of venepuncture and diagnosis were obtained and recorded in a data proforma.