INTRODUCTION Family planning is the conscious effort made by a couple and individuals to decide freely and responsibly on the number and spacing of their children. Contraceptives are methods used by couples to achieve a desirable family size and spacing. Many methods are available which include both the traditional and the modern methods. DMPA is a long acting reversible hormonal contraceptive that is given every three months. It is easily administered, and not coitus dependent. This has increased the awareness and subsequent uptake of the method by several women. However this increase in use has generated a lot of interest especially on the effect of DMPA on the metabolism of the body that is glucose, lipid, and protein metabolism. The effects of DMPA on the total serum protein,albumin and globulin have been documented in Asians and Caucasians but yet to be studied in recent time amongst Nigerian women hence this research. AIMS AND OBJECTIVES The aim of this study was to determine the effect of Depo medroxyprogesterone on the serum levels of total proteins, albumin and globulins of women in Adeoyo Maternity Teaching Hospital, Ibadan. METHODOLOGY It was a Prospective Cohort study involving fifty women who have consented to taking DMPA for the first time at the family planning clinic at the Adeoyo Maternity teaching Hospital. The participants were sequentially selected and recruited following informed consent. A structured proforma form was used to obtain Sociodemographic data , Bodymass Index BMI, weight. Serum proteins, Albumin and Globulins were measured before injection, one and three months after injection using standard enzymatic methods.