INTRODUCTION: Hypertensive disorders of pregnancy are important contributors to maternal and perinatal morbidity and mortality. The occurrence of significant proteinuria further worsens outcome. The 24-hour urine collection for assessment of proteinuria is the gold standard but it is time consuming, inconvenient and cumbersome for patient and the health care team. Dipstick urinalysis for assessment of proteinuria is challenged by high rates of false positive and negative results. It is not known if urinary collection for a shorter period will be as reliable as the 24-hour urinary protein assessment. OBJECTIVES: This study was aimed at determining the correlation between four-hour and 24-hour urinary protein estimation in women with hypertensive disorders of pregnancy at OAUTHC. METHODOLOGY: A cross-sectional analytical study. A total of 154 pregnant women with hypertension in pregnancy at gestational age of ≥20 weeks were recruited into the study. They had urine specimens collected in two separate containers labeled A and B over four hours and then the following 20 hours respectively. The protein content of sample A and mixture of sample A and B (24-hour urine) were determined by turbidimetric method. The protein values were then compared using the Receiver Operating Characteristic curve.