BACKGROUND Preeclampsia has a significant global burden, as it accounts for a number of maternal death and near misses as well as premature deliveries and fetal growth disorders. Preeclampsia has been shown to be more prevalent among Africans. Dysregulation of Nitric oxide (NO) which characterizes the widespread endothelial dysfunction seen in ppreeclampsia is believed to play a role in the aetiopathogenesis of various disorders in pregnancy. However Data on the biosynthesis of NO and its role in the pathogenesis of preeclampsia or, its effect has attracted divided opinion and remain unsettled. A meta-analysis of the available studies concluded that there is further need to study the role of Nitric Oxide and its’ metabolites in the pathogenesis of preeclampsia particularly in the developing countries where literature on the subject matter appears scarce. AIM The aim of this study was to compare the level of Serum Nitrite in normal pregnancy and pregnancy complicated with preeclampsia. OBJECTIVES The objectives of this study were to determine and compare the mean level of serum nitrite in pre eclampsia and normotensive pregnancy as well as to establish if there was an association between mean arterial pressure and serum nitrite in preeclamptics. METHODS This study was a case control study involving 100 women who were attended to at the department of Obstetrics and Gynaecology of University of Abuja Teaching Hospital. Fifty women with preeclampsia were matched with 50 women with normotensive pregnancy to determine their level of serum nitrite which is a proxy for NO. The Griess diazotization reaction was used to detect nitrite and nitrate formed by the spontaneous oxidation of NO under physiological conditions. The participants were recruited from the Obstetric emergency unit and the antenatal clinic of the department. Data was analyzed using Statistical Product and Services Solution (SPSS version 24). The level of significance was 95% with P value < 0.05.