Background: Chlamydia trachomatis is the commonest sexually transmitted disease that is capable of causing tubal pathology. Many women suffered from the infection unknowingly due to its asymptomatic nature, and are now presenting with complications such as; chronic pelvic pain, ectopic pregnancy and tubal infertility. This study was performed in four selected health institutions within the Jos Metropolisfrom May, 2014 to December, 2014. Objectives: To compare the prevalence and serum levels of IgG anti-Chlamydia trachomatis antibody in women with tubal infertility and fertile women in Jos. Methodology: This was a comparative study that was achieved through a casecontrol study. The study group comprised of 80 women with tubal infertility reported by HSG, these were compared with 80 women (controls) matched for age that attended six weeks postnatal care clinic.A consecutive sampling technique was used for both the cases and the control group. The subject’s history including sociodemographic factors, obstetric history, sexual and reproductive risk factors was obtained using a study pro-forma. Two milliliter of venous blood was collected from the subjects to determine the prevalence and serum levels of IgG anti-Chlamydial antibody among the cases and controls using the DRG Chlamydia trachomatis IgG ELISA kits. Statistical analysis: The collected data was compiled, tabulated and analyzed using statistical package for social sciences (SPSS incorporated Chicago version 16.0) software.Comparison of prevalence and the serum levels between the cases and control groups were carried out using matched paired Chi Square (McNemars) test and Wilcoxon signed rank test respectively. P- Value <0.05 was used as significant level. Receiver Operating Characteristic curve was used to determine the predictive value of chlamydia IgG antibody titre to determine tubal blockage.