INTRODUCTION Group B Streptococcus (GBS) is a Gram positive organism that is a major cause of bacterial infections in the peri-natal period. These include amnionitis, urinary tract infections and endometritis. At birth, 50-60% of the neonates born to colonized mothers have positive cultures taken from mucus membranes and the skin. About 98% of the colonized newborns remain healthy but 1-2% develops invasive disease. Published information about the prevalence of colonization by GBS among pregnant women in Northern Nigeria is scarce. Aim To determine the risk factors for GBS colonization, drugs sensitivity pattern of group B Streptococcus colonization among pregnant women at 35-37 weeks gestation and to compare the maternal and neonatal outcomes among GBS positive and GBS negative women within 7 days postnatal. Methodology The study was conducted in the antenatal clinic/pre-natal ward. labour ward, post-natal clinic and the medical microbiology unit of the Usmanu Dan-fodiyo University Teaching Hospital (UDUTH), Sokoto. This was a cross-sectional analytic study that involved pregnant women between 35-37 weeks of gestation who met the inclusion criteria and their neonates. A simple random sampling (by YES or NO balloting) of pregnant women who meets the inclusion criteria carried out. The study subjects were divided into GBS positive and GBS negative group and followed up to 7 days post-delivery with their newborns to determine the maternal and early neonatal outcomes. Vaginal and rectal swabs were collected and transported to the medical microbiology laboratory of Usmanu Danfodiyo University Teaching hospital, Sokoto, where they were processed. The cultures were done within 24 hours of collections, and cultured for growth of Group B Streptococcus and then tested for drugs susceptibility pattern.