Background: Infection withCytomegalovirus (CMV) especially in pregnancy may cause pregnancy complications such as congenital infection, non-hereditary deafness, intrauterine growth restriction and other skull deformities. Aim: The aim of this study was to identify risk factors for Cytomegalovirus infection and pregnancy outcome among infected pregnant women in Aminu Kano Teaching Hospital Studydesign: Thiswas a prospective cohort study. Method: This study enrolled 110 pregnant women attending ANC in Aminu Kano Teaching Hospital with age range between 19-42 yrs who were monitored till delivery. Blood samples were collected from each of 110 consecutive pregnant women attending the antenatal clinic of the hospital. Samples were analyzed for CMV specific IgG and IgM using EIA test kits. Those that were positive for both IgG and IgM were assayed for PCR to determine active infection. A semistructured researcher administered questionnaire was used to obtain socio-demographic information which included; age, socioeconomic class, parity and gestational age. To assess the neonatal CMV infection, newborns were examined by the researcher along with a Neonatologist. The data were analyzed using SPSS version 20 software package (IBM SPSS Statistics Inc., IL, Chicago USA). Measured variables were expressed in descriptive statistics; quantitative variables were summarized using range, mean and standard deviation while qualitative variable was presented in frequency and percentage.Chi-squared and Fisher’s exact tests were used to test for statistically significant association between categorical variables. A p value of < 0.05 was considered statistically significant. Also multivariate binary logistic regression model was also used to control potential confounders.