Aim of study The aim of the study was to assess retinal nerve fibre layer (RNFL) thickness measurements of normal Nigerian adults using Optical coherence tomography. Subjects and methods Ethical approval was obtained from the Review Board of Aminu Kano Teaching Hospital, Kano. The study was carried out in the Department of Ophthalmology of Aminu Kano Teaching Hospital between April 2010 and January 2011. One hundred and ten subjects (220 eyes) aged between 18 and 51 years were examined in the study. Visual acuity assessment was done using an illuminated Snellen’s chart, anterior segment examination was done using a slit lamp biomicroscope while fundus examination was done using a Keeler Professional Direct Ophthalmoscope. The Optical coherence tomography procedure was carried out with the Carl Zeiss Stratus OCT Model 3000 software version 4.0. The Fast RNFL scan protocol was used and a circular pattern of 3.4mm diameter was centred around the optic nerve head and scans were obtained.