BACKGROUND. Lower urinary tract symptoms secondary to benign prostatic hyperplasia (BPH) is a common urologic disease in aging men. The growth of the prostate is age and androgen dependent. The objective of the study was to determine the relationship between serum level of dihydrotestosterone (DHT) and prostate volume among patients with symptomatic benign prostatic enlargement. PATIENTS AND METHODS. The study was a cross sectional study conducted over a period of one year. Patients who fulfilled the inclusion criteria and willingly gave consent to participate in the study were enrolled. A total of 168 patients were evaluated for their symptomatology and physical examination findings. In addition to which their serum levels of dihydrotestosterone was assayed and prostate size measured with transrectal ultrasonography. Other investigations as applicable to patients being investigated for BPH according to the unit protocol were carried out. Data was analysed using Statistical Programme for Social Sciences (SPSS) version 21 and presented in the form of simple percentages, frequency tables and charts and the Pearson’s correlation coefficient(r) calculated and multiple linear regression done. P value <0.05 was considered statistically significant. RESULTS: The mean age of the study population was 63.04 years (+/- 8.82) and the mean serum DHT was 1.42 nmol/L (+/- 1.40). The Pearson correlation coefficient (r) between DHT and prostate volume was -0.161 (p = 0.04) showing a statistically significant relationship and that between age and DHT was -0.321 (p = <0.01). CONCLUSION: A statistically significant negative correlation was found to exist between serum dihydrotestosterone and prostate volume. Keywords: serum dihydrotestosterone, prostate volume, benign prostatic hyperplasia.