Sickle cell anaemia (SCA) is the most common genetic disease worldwide.It is characterised by reduced half-life of the erythrocytes due to repeated haemolysis and occlusion of the vessels referred to as sickle cell crisis. With increasing frequency of crises, the bone marrow becomes more active in order to replace the destroyed red blood cells. This depletes the minimal tissue stores of folate, causing a higher likelihood of folate deficiency.As a result of this, children and adults with sickle cell anaemia receivefolate supplements to restore the depleted folate levels and aid in production of erythrocytes. The outcome of the study might indicate the need or otherwise for additional folate supplementation during sickle cell crisis. The study aimed to determine red cell folate levels of children with sickle cell anaemia in steady state and during crisis attending University of Nigeria Teaching Hospital (UNTH), Enugu, and compare with controls. It was a hospital based, comparative, and cross-sectional study which was conducted over a seven-month period extending from September 3, 2018 to March 25, 2019. Fifty children with sickle cell anaemia were recruited during crises and followed up until they fulfiled the criteria for attaining steady state, when their blood samples were collected for repeat erythrocyte folate estimation. The controls were fifty children who were matched with the subjects for age and gender and had haemoglobin AA phenotype following haemoglobin electrophoresis done at recruitment. A pretested questionnaire was administered to the subjects and controls by the researcher, while subjects were clinically examined to determine the degree of liver and splenic enlargement. Blood samples were taken for red cell folate estimation using the automated Roche Cobas e411 equipment. Full blood count was also estimated using a 5-part automated Mindray BC-5300 equipment.