The neonatal period, (the first 28 days of life) is critical to a child’s survival. Every day, about 7000 newborn babies die world-wide and neonatal sepsis (NS) alone contributes to 18% of such deaths. The global mortality burden from NS is majorly contributed by Sub-Saharan Africa, as well as Southern and Central Asia. Nigeria has been shown to rank sixth among countries with the highest burden of neonatal deaths with NS alone contributing to 23-30% of such deaths. About 75% of such deaths can be prevented through high-quality care, which includes early diagnosis and prompt institution of appropriate treatment of NS. The gold standard for the diagnosis of NS is blood culture. This has a low yield, consumes time, and is cumbersome, thus creating an impetus for the search for an ideal biomarker that will aid in the early and quick diagnosis of sepsis. This study aimed to determine procalcitonin (PCT) levels in the diagnosis of NS. It also aimed to determine the relationship between PCT level and bacterial blood culture of neonates being evaluated for sepsis. It further sought to determine the sensitivity, specificity, positive, and negative predictive values of PCT in diagnosing NS.