Although the major basis of complications resulting from human immunodeficiency virus (HIV) infection is destruction of helper T-cells; the virus is also associated with many abnormalities of B-cell function resulting in the production of a large variety of autoantibodies that include those directed towards cardiolipin and those responsible for lupus anticoagulant positivity. The purpose of this study was to examine the prevalence of LACs in HIV-infected Nigerians and to evaluate an association of this antibody with some clinical and laboratory features of HIV/AIDS. The importance of ESR in the investigation of HIV/AIDS was also investigated. A total of one hundred and fifty five adult Nigerians consisting of 104 HIV-seropositive HAART naïve patients and 51 apparently normal, age and sex matched controls were enrolled after informed consent was obtained from each of the participants. Patients’ history and full physical examination was conducted and findings documented accordingly. All the participants were investigated with respect to lupus anticoagulant test (aPTT, KCT, PT), Complete Blood Count, Direct Coombs’ test, and Bone Marrow Aspiration (for patients only). Ninety-two (88.5%) of the patients fulfilled the CDC criteria for AIDS and twelve (11.5%) did not (non-AIDS). Three out of 104 (2.9%) patients and one out of 51 (1.9%) controls were positive for LAC. The difference was not significant, p = 0.973. Th positive correlation between the CD4 levels and KCT of patients (p = 0.031), but inversely correlated in than that of the controls (20.39 ± 17.62 mm/hr), p < 0.0001. The Marrow aspirates were either normocellular or hypercellular; forty-five out of 84 (53.6%) of the marrow aspirates had dyserythropoiesis, whilst the rest 39 (46.4%) had no observable bone marrow abnormality. The Perls’ stain for iron was negative in 31 (36.9%) patients. None of the subjects studied were DCT positive, neutropenic or lymphopenic. There was no correlation between the presence of LAC and anaemia, reduced platelets level (but still within normal for Nigerians), elevated ESR, dyserythropoiesis and marrow iron depletion. The present study demonstrates that LAC in HIV-infected patients is not associated with antiphospholipid syndrome.