BACKGROUND: Asymptomatic significant bacteriuria in pregnancy is a major obstetric risk factor, if left untreated. It is associated with poor maternal and perinatal outcomes. Evidence from research has confirmed the cost-effectiveness of early screening and treatment of asymptomatic significant bacteriuria in pregnancy. OBJECTIVE: To determine the prevalence, bacteriology, antibiotic sensitivity of isolates and predictors of asymptomatic significant bacteriuria among pregnant women attending the antenatal clinic at JUTH. METHODOLOGY: This was a clinic-based cross-sectional study involving 250 eligible pregnant women attending the antenatal clinic at JUTH. The women were selected using a systematic sampling technique. Data were collected from cultures of two clean-catch urine specimens and a third culture to ascertain clearance of asymptomatic bacteriuria after antibiotic treatment. Data were analyzed using Epi-info 2007 (version 3.4.1) statistical software. Tests of association between variables were performed using chi- square and t-test for categorical and continuous variables respectively. A pvalue of 0.05 was taken as significa