Background: IVF is the most sophisticated and effective treatment for infertility. Ovarian response to gonadotrophin stimulation is crucial for successful IVF outcome. Presently there is no consensus on the best predictor of ovarian response between AFC, ovarian vol, day 3 FSH and maternal age. Objective: The current study was designed to compare the prediction of ovarian response during IVF treatment by AFC, ovarian volume, day 3 FSH and maternal age. Methods: This was a prospective cohort study in a university teaching hospital assisted conception unit involving 222 women undergoing IVF treatment. All patients had transvaginal sonography to measure the basal ovarian volume and basal antral follicle count on day 3 of the treatment cycle. Basal FSH was assayed prior to the treatment cycle. In all patients, the test was followed by a standard IVF treatment. The result of controlled ovarian hyperstimulation during IVF treatment, expressed by the total number of follicles and oocyes retrieved, was used to assess ovarian response.