Preterm premature rupture of fetal membranes(PPROM) is a significant contributor to poor obstetric outcome. The need for a continuous search for the aetiological factors to enable its prevention and proper management if it occurs cannot be overemphasized. This was a case control study (73 cases versus 73 controls with on going pregnancy without rupture of membranes) aimed at determining the microbiological pattern of of PPROM in our environment. Endocervical culture of cases and controls were compared. The cases of PPROM had significantly more positive cultures than controls( 97.3% versus 5.5% ; p <0.001). Escherischia coli(32.8%) was the commonest organism isolated. Other organisms of statistical significance isolated include Proteus(21.9%), Bacteroides(15.1%), and Klebsiella(9.6%). These organisms showed very good sensitivity to Ciprofloxacin(90.4%), Ceftriaxone(89%), Ceftazidime(86.3%), Co-amoxiclav(80.8%), Erythromycin(73.9%), Cefuroxime(73.9%) and Gentamicin(65.8%). Clindamycin(45.2%) only showed moderate sensitivity but Cotrimoxazole Ampicillin, Amoxicillin and Cloxacillin all had low sensitivity. A significant number of PPROM(58.9%) occurred at 31 – 33 weeks gestation(P. value 0.014). Low level of education or low socioeconomic class was significantly associated with PPROM(82.2%, P <0.001) A combination of Cefuroxime and Erythromycin given intravenously for the first 48 hours followed by oral combination of these two drugs for a further 5 days is suggested based on the sensitivity pattern of the isolated organisms in this study. It is recommended that prophylactic use of antibiotics in the management PPROM should be based on the microbiological pattern and their sensitivity in any environment.