Background Dyslipidemia (DLP) is the third component of metabolic syndrome, a well-known cardiovascular risk factor. There are novel evidences on the association of DLP in pregnancy and gestational diabetes mellitus (GDM), however, the different lipid constituents during pregnancy and their changes with gestational age have not been well described. Objective To determine the relationship between gestational diabetes mellitus and abnormal maternal fasting plasma lipid constituents Methods A total of 114 women were enrolled for this case-control study (36 cases and 78 controls) from May 2018 to December 2018. Relevant sociodemographic and anthropometric information was obtained at first contact. Plasma samples for oral glucose tolerance test and fasting lipid measurements were obtained between 24 to 28 weeks, and assayed using the glucose oxidase method and modified LiebermannBurchards reaction, Phosphotungstic acid and magnesium chloride methods. Results obtained were analyzed using Statistical Package for Social Sciences (SPSS) version 21 (IBM SPSS Armonk, NY: IBM Corp). Categorical variables were presented in percentages while continuous variables were expressed as means (±Standard Deviation). Student t-test and Chi-square test or Fishers exact test were used for comparing variables between the two groups. A p-value of <0.05 at 95% confidence interval was considered statistically significant.