BACKGROUND Malaria and helminth infections are endemic in sub Saharan Africa and contribute significantly to anaemia and other morbidities during pregnancy such as intrauterine growth restriction and low birth weight. The co-infection of malaria and helminth in pregnancy has more negative impact on the prevalence of anaemia in pregnancy. While proactive measures are taken to prevent malaria and its sequelae during pregnancy at the University of Port Harcourt Teaching Hospital, there are no such measures for helminth infection even though it is equally endemic and have adverse sequelae like anaemia. The diagnosis of helminth infection unlike malaria in pregnancy is rarely made at University of Port Harcourt Teaching Hospital. OBJECTIVE The main objective of this study was to determine the prevalence of malaria and helminth co-infection in pregnancy and its effect on anaemia in pregnancy at booking, in the University of Port Harcourt Teaching Hospital. METHODOLOGY This cross sectional descriptive study was conducted among 192 pregnant women booking for ante-natal care at the University of Port Harcourt Teaching Hospital from August 2015 to March 2016. All pregnant women booking for ante-natal care who met the eligibility criteria were enrolled for the study. Informed consent was obtained from all pregnant women that took part in the study. Sociodemographic data was collected through a structured questionnaire. These data included age, level of education, occupation, marital status, area of residence, religion and parity. Blood samples were collected and evaluated for haemoglobin estimation and malaria parasite, stool samples were examined for ova of helminth. Data was statistically analyzed using SPSS for windows version 20.0. The analyzed data were presented in tables and chart.