This was a descriptive cross-sectional study carried out at the Emergency Paediatric Units of Jos University Teaching Hospital and Bingham university Teaching Hospital, Jos, northcentral Nigeria. One hundred and sixty consecutive under-five children who presented with signs and symptoms consistent with H. influenzae infection were recruited. Socio-demography data was obtained with structured questionnaire. Specimens including cerebrospinal fluid, blood, ear, throat and eye swab were collected and carefully processed for isolation of H. influenzae. The specimens were macroscopically and microscopically examined before the specimens were cultured in Sheep chocolate agar. The isolates were identified by satellitism and X+V factor requirements and were biotyped using indole, urease and ornithine decarboxylase biochemical tests and serotyped by slide agglutination test. Antibiotics susceptibility pattern and minimum inhibitory concentration of the isolates determined by disk diffusion method and Etest respectively. The prevalence of H. influenzae was low in Jos with prevalence of 6.3% among 160 under-five children studied, three isolated from JUTH and seven from BUTH, with 1 mean age of 34 months and M: F ratio of 1:1. About 60% of these isolates were obtained from patients with acute pyogenic bacterial meningitis and H. influenzae were isolated predominantly from children whose parents were Farmers and Artisans. Most of the isolates were biotype 1 and fifty percent of the isolates were serotype b. Majority of the isolates were ampicillin resistance, β-lactamases producers and were all sensitive to ceftriaxone and azithromycin. The incorporation of vaccination against H. influenzae into the national immunization schedule is advised because of the risk of complications.