This study was carried out to assess the utility of various indices of sepsis screen in the Neonatal Intensive Care Unit (NICU) of the Obafemi Awolowo Teaching Hospital Complex (OAUTHC) Ile-Ife. Bacterial and haematological profile of these neonates was studied. Parameters of sepsis screen such as leucocyte count, I/T ratio, I/M ratio, degenerative changes in neutrophils, micro ESR and test for C reactive protein (CRP) were determined. Over a period of 14months, 70 neonates with clinical and laboratory evidence of septicaemia were recruited into the study after obtaining consent from their mothers. 6ml of venous blood was collected for haematological (haematocrit, leucocyte count, peripheral blood film examination, platelet count, micro ESR), immunological (CRP) and microbiological (blood culture) tests. Data collected was analysed using descriptive and inferential statistics; a p-value <0.05 was significant. The results showed that the study population consisted of 44(62.6%) males and 26 36.4% female with mean (+ SD) ages of 5.6 +4.3 and 4.5 +3.7 days and mean (+SD) weight of 3.03 +0.62 kg and 3.0 + 0.61kg respectively. The commonly observed symptoms were vomiting 82.9% and refusal of foods 31.4%. While the signs found in them included respiratory distress 70%, jaundice 11.4% and hyperthermia. The enterobacteriaes and both coagulase positive and negative staphylococcus were the common isolates. Although, there were statistical differences in the mean (+SD) of haematocrit, total leucocyte count, absolute neutrophil count, I/T ratio, I/M ratio, and platelet count, they were within the normal range and since the normal values for the subjects were not known, it thus make it difficult to consider them as diagnostic parameters in neonatal septicaemia from this study. The micro-ESR has a positive correlation with the CRP and this has reaffirms the continued relevance of the use of micro-ESR for the assessment of acute phase response. Degenerative changes were present in the neutrophil. However, thrombocytopenia was not a common finding in these neonates. Therefore, since there is a clear correlation between micro ESR and the CRP, the micro-ESR still continue to have diagnostic relevance in neonatal septicaemia in this environment.