Background: Glucose-6-phosphate dehydrogenase (G6PD) is a key regulatory enzyme in the hexose monophosphate shunt with the major role of generating reduced nicotinamide adenine dinucleotide phosphate (NADPH) to meet cellular needs for reductive biosynthesis and maintenance of the cellular redox status. G6PD deficiency is a common inherited enzyme disorder of mankind known to be associated with severe neonatal hyperbilirubinaemia that can result in permanent neurologic damage or death. This study was aimed at estimating the level of G6PD activity among icteric neonates, identifying G6PD deficient neonates, other associated risks factors for development of hyperbilirubinaemia and its attendant complications in Jos. Patients and Methods: One hundred and fifty icteric neonates made up of 92 (61.3%) males and 58 (38.7%) females whose parents consented were consecutively enrolled as they presented at the Special Care Baby Units (SCBU) of the Jos University Teaching Hospital (JUTH), Bingham University Teaching Hospital (BhUTH), and the Plateau State Specialist Hospital (PSSH), Jos, from March 2013 to February 2014. These subjects had their G6PD activity levels assayed using the Pointe Quantitative Diagnostic Kit (USA). In addition, relevant clinical information was obtained using a questionnaire, while relevant laboratory investigations were carried out on the neonates and their mothers.