Background: Genital colonization by group B streptococcus (GBS) in pregnant women in their third trimester has been shown to be a known risk factor for morbidity and mortality among new-borns. Aim To determine the prevalence of GBS colonization among pregnant women at FMC Abeokuta, evaluate its associated socio demographic factors and determine Neonatal outcome among exposed babies. METHODOLOGY This was a longitudinal cohort study. The study was carried out in the ante-natal clinic and labour ward of the Department of Obstetrics and Gynaecology of Federal Medical Centre, Abeokuta. One hundred and sixty pregnant women presenting for routine ante-natal clinic between 35-41 weeks gestational age that consented to the study were recruited consecutively. Two vagina swab samples were taken from each participant under aseptic condition with a disposable speculum and transported immediately to the Medical Microbiology Laboratory of FMC Abeokuta. Swab samples were processed using standard microbiological methods. Babies whose mothers were positive were observed to detect those with features of early onset Neonatal sepsis and screened for evidence of infection in conjunction with the paediatrician using C-reactive protein kit (fisher sure-vue CRP kit). Main outcome The main outcome measured were the presence of GBS infection among pregnant women and the neonatal outcome which included neonatal sepsis, meningitis and pneumonia.