Aim and objectives: This cross-sectional study was carried out to determine the fungal conjunctival flora of patients without anterior segment diseases at the Guinness Eye Center, Onitsha, Nigeria. Materials and method: New adult patients attending the Guinness Eye Center, Onitsha were recruited using simple random sampling technique. Conjunctival specimens were taken from the inferior conjunctival sac of the right eye using a sterile swab stick (sterillin, Lot no. 120308) and immediately transported to the laboratory. Specimens were inoculated unto the surface of Sabouraud dextrose agar slant in test tubes and incubated at room temperature (27˚C). The tubes were examined for growth every 2 days for 4 weeks after which it was discarded if there was no growth. A small piece of the mycelial mat was removed from the agar surface using a straight wire loop and teased in a drop of lactophenol cotton blue stain on the surface of a clean glass slide. The preparation was covered with a glass cover slip and viewed under the microscope using x10 and x40 magnifications. The identity of the 2 fungus was also determined from its morphology. Results: Samples from two hundred and twenty five patients were studied. Sixty two (27.6%) had positive fungal culture. Aspergillus specie was the commonest organism (n=26, 41.9%) followed by Candida specie (n=16, 25.8%). Majority of the patients whose samples grew fungi were farmers (n=22, 35.5%) and traders (n=11, 17.7%). Conclusions: 1. More than 25% of patients with apparently healthy conjunctiva and anterior segment habour fungi some of which are pathogenic. 2. Aspergillus specie is the commonest fungal isolate among patients followed by Candida specie. 3. Most of the patients whose samples grew fungi were farmers followed by traders.