Background: Glaucoma is the leading cause of irreversible visual impairment in the world affecting 60.5 million people worldwide in 2010, which is expected to increase to approximately 76.0 million by 2020 and 111.8 million in 2040. Among other solutions to curbing this blinding effect of the disease, will be early detection and monitoring but this however requires expensive technology and or well-trained personnel before an accurate diagnosis of the disease can be made. Therefore, it’s important, to use the Mars CS chart which is a fairly quick and relative inexpensive test to asses our glaucoma subjects and controls to see if there is an association between CS and glaucoma. AIM: To evaluate the use of contrast sensitivity in evaluating glaucoma patients and assessing factors that may influence contrast sensitivity in these patients and normal control subjects. MATERIALS AND METHODS: This was a hospital based cross sectional, comparative descriptive study conducted from 1st October 2020 to 30th April 2021 at the Benue State University Teaching Hospital, Makurdi LGA, Benue state, Nigeria. Fifty consenting consecutive, glaucoma patients who met the inclusion criteria were recruited from the glaucoma clinic, while 50 age-matched controls were recruited from other clinics. All participants had a detailed history taken with their best corrected visual acuity, anterior and posterior segment examinations. They all subsequently had their central visual field done using the Humphrey (Tomey AP – 250; version 7.4.0) and then contrast sensitivity(CS) test performed using Mars contrast sensitivity chart (MAR USAR). All subjects’ information was inputted into a standardized questionnaire. RESULTS: The participants age range was 21 to 69 years with mean age of 44.2 (± 13.13) years. There were equal males and females (M: F = 1:1). The mean of CS (1.67±0.09) in xiv control group was higher than that in glaucoma (1.40 ±0.17) which was statistically significant (𝑝 <0.001). The glaucoma cases were further sub grouped based on disease severity using mean deviation of visual field with results indicating a progressive reduction in the mean CS in mild glaucoma (1.58 ±0.17) compared to moderate (1.44 ±0.11) and severe (1.29 ±0.20) which was significant at a P- value of <0.001. Ocular parameters like BCVA, CDR, and MD of visual field varied significantly (p< 0.05) between the glaucoma and control groups while IOP and pupillary size did not differ significantly. The MD of visual field perimetry, VA and CDR correlated well with the CS test in glaucoma patients (r = -0.32, 𝑝= 0.001; r = -0.33, 𝑝= 0.001; r = -0.41, 𝑝< 0.001 resp.). There was a strong negative correlation with age and CS in normal subjects (r = -0.60, 𝑝< 0.001) while VA (r = -0.29 𝑝= 0.003) and CDR (r = 0.23 𝑝= 0.023) showed weak correlation with CS.