Background: Both short and long inter-pregnancy intervals have been widely associated with adverse obstetric outcomes. The WHO recommended a minimum of 24 months inter-pregnancy interval to reduce the risk of adverse maternal and fetal outcomes. However, inter-pregnancy interval practices in many developing countries including Nigeria remain minimally addressed. Objective: To determine the effect of inter-pregnancy interval on serum ferritin and haematocrit levels and pregnancy outcome among parturient in University of Ilorin Teaching Hospital, Ilorin, Nigeria. Study design: A prospective cohort study of parturient less than 20weeks gestation. Those who did not satisfy the WHO recommended inter-pregnancy interval of at least 24months were categorized as group II while gestational age and social status matched parturient who satisfied the WHO recommendation were in group I. Setting: Antenatal booking clinic of University of Ilorin Teaching Hospital, Ilorin, Nigeria. Methodology: A total of 316 parturient who satisfied the inclusion criteria were recruited for the study by systematic sampling. These were equal number of 158 participants each as subject and control. Subject and control were matched for gestational age and social status. The serum ferritin and haematocrit levels as well as pregnancy outcomes were evaluated for each participant. The results were analysed using SPSS version 20.0 with appropriate tables and figures generated.