Objective: To determine the prevalence of dry eye disease (DED) among glaucoma patients on topical ocular hypotensive drugs and compare the prevalence to age and sex matched non glaucoma cohorts. To identify the independent predictors of dry eye disease in both groups in patients presenting to ophthalmic clinic in Federal Medical Centre, Umuahia, Abia State, Nigeria, in order to effect an improved patients care. Materials and Methods This was hospital -based observational case -control study, in which a total of 350consecutive consenting adult patients aged 30 years and older presenting with primary open angle glaucoma on topical medication for up to one year and non -glaucoma age/sex matched controls not on any topical medication, were assessed for dry eye disease. Duration of study was 13 months. Assessment was done subjectively with validated questionnaire – Ocular Surface Disease Index (OSDI), and objectively with Schirmer’s test and tear film breakup time (TBUT). An OSDI score of > 12, TBUT - an appearance of first dry spot around the central cornea in less than 10 seconds, a Schirmer’s test reading of<10mm wetting in five minutes were considered pathological and indicative dry eye disease. Data generated from the study was entered into a laptop computer and analyzed using the SPSS version 20. Statistical analysis was made using both the descriptive and inferential statistics including Chi square (x2) test; Pearson correlation and Student t-test with an alpha level at 0.05.