Background: Sexually transmitted infections (STIs) represent a major public health problem in developing countries including Nigeria. Millions of people especially women suffers from them. The prevalence of STIs has increased during the last few decades as the number of pregnancies per year is increasing. The super imposition of one factor on the other can be expected to amplify the effects of STIs on pregnancy and neonatal morbidity. However no such study has been published from this area. Objective: To determine the seroprevalence of HIV, HBV, and Syphilis infections among pregnant women making their first visit to the antenatal clinic of Kogi State Specialist Hospital (KSSH), Lokoja and also to determine the risk factors associated with these infections. Design: It is a cross sectional study. Setting: Department of Obstetrics and Gynaecology, KSSH, Lokoja, Kogi State. Methods: Three hundred pregnant women attending the antenatal clinic for the first time were recruited for the study between January 1 and December 31, 2016 and were screened for HIV, HBV and syphilis. Their demographic data, risk factors and results of the screening tests were analysed and presented in simple charts, tables and percentages.