Background: Seminal plasma zinc level is significantly correlated with sperm concentration, motility and morphology. Abnormal level of this essential trace element has been associated with abnormal semen parameters. Routinely, this element is not assessed during investigation of male factor infertility. With its important role in normal testicular development, spermatogenesis and sperm motility, assessment of zinc levels during evaluation of male partners of infertile couples and possibly replacement therapy may improve abnormal sperm parameters. Objective: To determine the levels of zinc in seminal plasma and its relationship with sperm quality in normozoospermic and oligozoospermic males. Study Design: A comparative analytical case control study of male partners of infertile couples presenting for infertility assessment. Methods: Semen parameters of 116 healthy consented male partners of infertile couples oligozoospermia (n=59), normozoospermia (n=57) undergoing evaluation for infertility were assessed. Seminal plasma zinc levels were determined using Flame Atomic Absorption Spectrophotometer. Zinc concentration was expressed as mol/L. Primary outcome measure was zinc levels in seminal plasma in oligozoospermic men. Data entry and analysis was performed using SPSS software for windows version 16.0. An independent t test was used to compare the scores of each of the measures and mean of parameters between the two groups. The Pearson’s correlation of coefficient test and Linear Regression were used to analysis and examine relationship between the semen parameters and zinc concentration scores. Test of significant was by chi square and Fisher exact test when appropriate and a probability level of ≤ 0.05 was considered statistically significant.