Background: Genital Chlamydia trachomatis is a common bacterial cause of sexually transmitted infection worldwide which is associated with significant morbidity and has a great impact on human reproduction and infertility. Infertility is a major health problem in Nigeria with a prevalence of between 20-40% compared with global prevalence of 15%. Objective: To correlate genital Chlamydia trachomatis infection with tubal blockage among infertile women and to compare with women attending family planning clinic in AKTH. Materials and Methods: This is a cross sectional prospective study that was carried out among 142 women with tubal infertility attending the gynaecological clinic and 142 fertile women from family planning clinic of AKTH. The women were consecutively recruited as they presented to the clinic until the desired sample size was achieved over a period of 12 weeks. A structured questionnaire was administered and related information and consent was obtained. Endocervical swab and blood samples were collected and tested using Eugene Chlamydial Rapid test Device and ELISA IgG Chlamydia antibody kits following manufacturer’s instruction.