Background: Bacterial contamination from conjunctiva of surgical eye patients may lead to infective endophthalmitis and identification of conjunctival isolates and their antibiotic sensitivity would help in planning appropriate prophylactic interventions. Aim and objectives: To determine the predominant conjunctiva bacterial flora in patients scheduled for cataract surgery at National Eye Centre Kaduna and their antibiotic sensitivity pattern. Materials and methods: The study comprised of 275 consecutive adult patients who underwent cataract surgery between January 2017 and April 2017 and met the inclusion criteria. Conjunctival swabs were obtained and placed on blood and chocolate media for isolation of bacteria. Bacteria found on the plates were then tested for susceptibility to Chloramphenicol, Ciprofloxacin, Ofloxacin, Levofloxacin, Imipenem, Cefuroxime, Ceftriazone, Ceftazidime, Amikacin, Gentamicin, Tetracycline and Penicillin. Epi Info 7 software was used for data analysis. Results: Bacterial growth was seen in 118 (42.9%) of the patients. The bacteria identified were: Staphylococcus epidermidis group (48.3%); Staphylococcus aureus (44%); Gram positive bacilli (6%) and Streptococcus species (1.7%). Maximum sensitivity was found with Imipenem, Cefuroxime, Gentamcin, Chloramphenicol, Amikacin and Levofloxacin. Maximum resistance with Tetracycline and Penicillin was also recorded. A statistically significant relationship was found between age and culture status (P = 0.0192) Conclusion: The predominant bacteria on the conjunctival sac were Staphylococcus epidermidis group and Staphylococcus aureus, which were sensitive to some commonly available antibiotics like chloramphenicol.