BACKGROUND: Cervical cancer is a preventable cancer. There has been a marked reduction of cervical cancer related mortality in high income countries, due to organised screening services using Pap smear cytology as a screening tool. In low income countries due to challenges in implementation of screening with Pap smear, there is a high morbidity and mortality rate from cervical cancer. To address this scourge, alternative screening methods such as Visual Inspection with Lugol’s Iodine (VILI) and Visual Inspection with Acetic acid (VIA) have been considered, however their validity must be continually assessed in different populations. OBJECTIVE: To compare diagnostic performance of VILI, VIA, and Pap smear cytology as screening tests for cervical cancer. METHODS: It was a comparative study, conducted in Amukpe, Sapele Local Government Area, of Delta State, Nigeria. Ethical approval was obtained from the Health Research Ethics Committee of DELSUTH. Participants were premenopausal women above 25years, who consented after counselling to participate in the study. Participants underwent the index and gold standard tests following this order: 1) speculum examination, 2) Pap smear sample collection, 3) Colposcopy assessment (at low magnification), 4) consecutive VIA with Colposcopy (at high magnification), 5) consecutive VILI with Colposcopy , 6) biopsy collection where indicated.The data collected were inputted into SPSS version 22 and analysed.