AIM: To compare the effect of different head positions on the intraocular pressure between patients with and without glaucoma attending the Eye Clinic in the University of Calabar Teaching Hospital (UCTH) Calabar. Materials and Methods: New patients aged >18 years who consented were recruited for the study. Each patient had ocular assessment >24 hours before the intraocular pressure measurements and included a visual acuity check, external eye examination, direct and indirect ophthalmoscopy. The intraocular pressures of each patient were measured using the Perkin’s handheld tonometer in the upright (sitting) position, in the horizontal (flat) position and in the 25° head-up position. Data were obtained using a questionnaire containing biodata, sociodemographic characteristics, characteristic relevant ophthalmology questions and measures of interest such as blood pressure, weight and height. Data were entered and analyzed using Statistical Package for the Social Sciences (SPSS) version 20.0 (SPSS Inc., Chicago, IL, USA). The significance level for association between variables was p< 0.05.