AIM: To compare refractive error measurements performed using the autorefractor with that of the focometer in patients with refractive errors with a view to determining the accuracy, validity and reliability of the focometer. METHODS: The study was a comparative cross-sectional study conducted among patients with refractive errors attending the Guinness Eye Centre Clinic of LUTH. Ethical approval was obtained from the Health Research Ethics Committee of Lagos University Teaching Hospital and the tenets of Helsinki declaration was strictly adhered to. Consecutively consenting patients who met the eligibility criteria were recruited until the sample size was attained. All participants had a standardized protocol examination including visual acuity assessment and ocular examination. The refractive error was measured using the autorefractor, focometer and subjective refraction. Statistical analysis for both the right and left eyes were done using IBM- Statistical Package for Social Science (IBM-SPSS, Version 21.0, IBM Corp. Armonk, USA). Frequencies, means and standard deviations were generated to observe the patterns of variable distribution among respondents. Comparison was done based on the means of variables of autorefractor, subjective refraction and focometer measurements using the paired-sample t-tests, Pearson’s correlation and linear regression. Agreement between the measurements was investigated using the Bland-Altman analysis and reliability of the repeated measurements tested with Cronbach’s alpha. The analysis was considered statistically significant when the p-value was <0.05.