Background: Premature rupture of membranes is an important obstetric condition that poses significant risk to the wellbeing of the foetus in-utero, constituting a source of morbidity and possible mortality if not appropriately diagnosed and managed. Approximately 8% to 10% of term pregnancies will experience spontaneous rupture of membranes prior to the onset of labour. An accurate diagnosis with prompt and correct intervention is of utmost importance in the management of rupture of membranes especially in preterm gestation. The search for accuracy and increased specificity in diagnosis of premature rupture of membrane is an ongoing process. Objective: To determine the accuracy of placental alpha micro globulin 1(PAMG 1) Amnisure ROM in diagnosing presence of amniotic fluid in cervicovaginal secretions and hence diagnose rupture of fetal membranes. To compare Amnisure ROM with standard clinical assessment in diagnosing rupture of fetal membranes. To determine and compare the sensitivity and specificity of amnisure ROM and standard clinical assessment in diagnosis of premature rupture of membranes. Methodology: All pregnant women with symptoms suggestive of premature rupture of membrane such as trickling or gush of fluid per vagina were evaluated using the amnisure ROM kit and standard clinical evaluation and findings noted on appropriate proforma. Data analysis: Data collected using the proforma was subjected to statistical analysis using SPSS version 18.