Background: Prelabor rupture of membranes is a common obstetric complication which present with some diagnostic challenge especially in equivocal cases. Standard methods of diagnosis had relied on history and clinical examination with the demonstration of pooling of liquor or its egress from the cervical os on speculum examination. These methods (including nitrazine and fern test) are however, limited by high false positives and negatives. In the absence of a non-invasive gold standard for diagnosis, several rapid, less invasive methods of diagnosis are being developed based on the demonstration of biomarkers with high concentrations in amniotic fluid, with increased levels observed in cervicovaginal fluids only with fetal membrane rupture. This study therefore compared the accuracy of these traditional methods and placental alpha microglobulin-1. Objective: To compare the accuracy of placental alpha-microglobulin-1 (PAMG-1) in cervicovaginal secretions with that of other standard methods in the diagnosis of prelabour rupture of membranes. Methodology: A longitudinal study from March to November 2011 conducted in women with symptoms and signs of prelabor rupture of fetal membranes. Standard diagnostic methods were carried out and in addition findings at PAMG-1 assay using the Amnisure ROM test were compared with the reported final diagnosis at delivery. The final diagnosis of membrane rupture was definitively made upon review of medical records after delivery and thereafter used to determine false positives and negatives. Descriptive analysis was done using Statistical Package for Social Sciences (SPSS) version 19.