Background Ovarian reserve (OR) is a function of the number and quality of the oocytes present in both ovaries. Among the available markers for the assessment of the OR, Anti-Mullerian Hormone (AMH) has been suggested as the most informative. However, there seems to be no clear-cut evidence to suggest that there is a difference between the serum AMH levels in fertile and infertile women. Therefore, the study seeks to evaluate any difference in the serum AMH levels between the two categories of women aged 18-35 years. Objective: To compare the serum Anti-Mullerian Hormone levels in fertile and infertile women and determine the average reference interval in the fertile group. Methodology: This study was a cross-sectional laboratory study of 47 infertile women and 47 fertile women matched by age at the Postnatal/Immunization and the Gynaecology clinics of the University of Abuja Teaching Hospital (UATH), Abuja. The infertile participants were recruited from the Gynaecology clinics. The fertile participants were enlisted from postnatal and immunization clinics at ≥ 6 weeks postpartum. Information on their socio-demographic characteristics,obstetric and gynecologic history was obtained with the aid of a proforma. Anthropometric measurements such as weight and height were measured using the scale mounted seca stadiometer at first contact. Venous blood samples for serum AMH levels were obtained from the fertile and infertile participants on any day of their menstrual cycles. Enzyme-linked Immunosorbent Assay (ELISA) method was used to assess the serum AMH levels for both groups. Consequently, the serum AMH levels of fertile subjects were used to establish the reference range. Data obtained were analyzed using Statistical Package for Social Sciences (SPSS) version 20. Continuous variables were expressed as means and standard deviations. The Student’s t-test was used for continuous variables while the Fisher-exactand Chi square tests were used for the dichotomous or categorical variables. Pearson’s correlation test was used to determine the relationship between Age, Body Mass Index (BMI) and AMH. A p-value of less than 0.05 was considered statistically significant.