Introduction/Background: Sickle Cell Anaemia (SCA) is a genetic disorder. The disability is lifelong and is of Public Health importance. The diversity in clinical and laboratory presentations may be due to additional genetic differences and environmental factors. The genetic factors may be in the β-globin gene itself, the β-globin gene cluster or elsewhere in the genome. Aim and Objectives: This study aims to characterize the β-globin gene for additional mutations associated with the Sickle Cell mutation. The study is to also determine the Haematological and Clinical features and to determine any relationships between these and the β-globin gene characteristics. Materials and Methods: This was a cross-sectional descriptive study utilizing questionnaires, physical examination, blood and buccal swab specimens. Fifty-one adult SCA patients participated. Full Blood Count and β-globin gene DNA Sequencing were carried out. The DNA sequences were compared with a Genbank Reference β-globin gene (NC_000011.9) using Basic Local Alignment Search Tool (BIntroduction/Background: Sickle Cell Anaemia (SCA) is a genetic disorder. The disability is lifelong and is of Public Health importance. The diversity in clinical and laboratory presentations may be due to additional genetic differences and environmental factors. The genetic factors may be in the β-globin gene itself, the β-globin gene cluster or elsewhere in the genome. Aim and Objectives: This study aims to characterize the β-globin gene for additional mutations associated with the Sickle Cell mutation. The study is to also determine the Haematological and Clinical features and to determine any relationships between these and the β-globin gene characteristics. Materials and Methods: This was a cross-sectional descriptive study utilizing questionnaires, physical examination, blood and buccal swab specimens. Fifty-one adult SCA patients participated. Full Blood Count and β-globin gene DNA Sequencing were carried out. The DNA sequences were compared with a Genbank Reference β-globin gene (NC_000011.9) using Basic Local Alignment Search Tool (BLAST) and variations noted. Data generated were analyzed using SPSS Version 17.0. LAST) and variations noted. Data generated were analyzed using SPSS Version 17.0.