Perinatal asphyxia is a clinical condition that may be associated with multisystemic complications inclusive of cardiovascular dysfunction. A proper evaluation of cardiovascular status with a view to ensuring early diagnosis of cardiac dysfunction and prompt intervention where necessary is a recognized approach in the management of asphyxiated neonates. Previous studies that evaluated cardiovascular status in this group of neonates using cardiac biomarkers were carried out predominantly in developed countries, despite the high burden of perinatal asphyxia in the developing countries. Furthermore, the relationship between the biomarkers and outcome measures, such as mortality have not been adequately described in Black populations. Thus, this study aimed to determine the serum levels of troponin I and brain natriuretic peptide (BNP); and their relationship with cardiovascular clinical parameters, disease severity, and survival in newborn babies with HIE. This was a comparative cross-sectional study. A total of 85 asphyxiated term neonates with HIE and an equal number of gestational and chronological age-matched healthy controls were recruited within the first 72 hours of life. The diagnosis of HIE was based on Sarnat and Sarnat staging. Socio-demographic, and other relevant clinical data were obtained from both groups. The cardiovascular status was assessed by clinical parameters and cardiac biomarkers (troponin I and BNP). Blood samples were collected between 24 to 72 hours of life to determine the level of troponin I and BNP using RayBio® human troponin I ELISA kit and BNP ELISA kit by WKEA respectively. The obtained data were analyzed using the SPSS 20.0 software package. The variable were presented and subjected to appropriate statistical test to determine their significance.