Background: Urinary tract infection (UTI) is the most common bacterial infection during pregnancy and this can be symptomatic or asymptomatic. UTI during pregnancy may cause complications such as pyelonephritis, hypertensive disease of pregnancy, anaemia, chronic renal failure, premature delivery and foetal mortality. Thus there is the need for its detection and treatment with safe and sensitive antibiotics to avoid maternal and perinatal mortality. Objectives: The study aims to determine the prevalence, aetiologic agents and antimicrobial susceptibility pattern of UTI among pregnant women in Enugu, Nigeria as well as to assess the accuracy of urine microscopy in screening for UTI in pregnancy using urine culture as the standard. Methodology: The study was done in the University of Nigeria Teaching Hospital, Ituku/Ozalla. It was cross-sectional in design and the research subjects were 233 consecutive symptomatic and consenting pregnant women out of the 1,652 women that were screened using symptoms of urinary tract infection. A pre-tested and anonymous questionnaire was administered individually to the sample population and their urine samples tested microscopically, cultured and then the antibiotics sensitivity tested.