Neonatal sepsis is among the leading causes of morbidity and mortality, particularly in the developing countries where the risk factors for the development of the disease are prevalent. The bacterial agents responsible for neonatal sepsis and their susceptibilities to antibiotics vary from time to time and from place to place hence the need for frequent update. This was a hospital-based descriptive cross-sectional study aimed at determining the prevalence of blood culture positive bacterial neonatal sepsis, the clinical features, bacterial isolates and their antibiotic sensitivity pattern as well as factors associated with neonatal sepsis at the Special Care Baby Unit (SCBU), Aminu Kano Teaching Hospital (AKTH), Kano. The study was carried out between September 2015 and August 2016. Three hundred and forty neonates with suspected sepsis were studied. Socio-demographic characteristics and clinical features of the neonates were obtained using a standard proforma. Blood culture was done on admission using the automated blood culture system (BACTEC) and bacterial isolates were identified using standard procedures. Antibiotic susceptibility testing was done using disc diffusion methods. The prevalence of blood culture positive neonatal sepsis was 28.8 per 1000 live birth. The infection rate was higher among the out-born (34.4%) than the in-born (27.0%) babies. The most frequent presenting complaints among the subjects were fever, difficulty with breathing and inability to suck. The symptoms significantly associated with sepsis were fever, difficulty with breathing, preterm birth and abdominal swelling (p < 0.05). The most frequent signs elicited among the subjects were pyrexia, respiratory distress, tachypnoea and tachycardia.