Background: Pre-eclampsia is characterized by oxidative stress and it is one of the major contributors to maternal mortality worldwide. There is yet no generally acceptable predictor or marker for pre-eclampsia, but it is becoming popular knowledge worldwide that serum selenium level drops significantly with pre-eclampsia. Selenium is mainly excreted in urine; it is therefore necessary to assay for selenium in urine which is non-invasive, other than the invasive venepuncture for serum assay. Objective: To assess the urine selenium level and serum Total Anti-Oxidant status of women with pre-eclampsia and compare it with women without pre-eclampsia. Methods: This research was a cross sectional analytical study. Urine and blood samples were collected from the participants. There were three groups of eighty women each. Group 1 comprised 80 women with pre-eclampsia, Group 2, 80 pregnant women with no form of hypertensive disorder, while the remaining 80 women were non-pregnant women attending family planning clinic at the two study centres- University College Hospital (UCH) and Adeoyo Maternity Teaching Hospital (AMTH) both in Ibadan. Urine selenium was measured using Atomic absorption spectrophotometry, while Total Anti-Oxidant Status was assayed for by spectrophotometric determination of Total AntiOxidant activity modified by Koracevic et al (2001). Relevant information about the participants and their diet were obtained by using questionnaire.