Background: The ovarian reserve and responsiveness to exogenous gonadotropins are important in the in-vitro fertilization and embryo transfer treatment cycle. Various markers have been used in assessing ovarian reserve but anti-mullerian hormone is the most recent. Objective: To evaluate basal anti-mullerian hormone (AMH) as a marker of ovarian reserve and responsiveness. Methodology: This was a cross-sectional study. Stored basal menstrual cycle day three serum samples of at least 138 women undergoing the in-vitro fertilization treatment cycle in a private fertility centre in Abuja was evaluated for anti-mullerian hormone and correlated with the number of oocytes retrieved. A Receiver Operating Characteristic was performed to determine the level of accuracy of AMH in predicting the ovarian reserve and responsiveness. Also, a reference range for low, normal and high response was determined for this subset of the Nigerian population.