Sickle cell anaemia (SCA) is a disease of global public health importance contributing to high childhood morbidity and mortality. Renal dysfunction including symptomatic and asymptomatic bacteriuria (ASB) usually starts in childhood. Children with ASB need to be identified early to forestall disease progression and attendant consequences. Jos that has peculiar conditions that predispose to accentuated morbidities in children with SCA lack current data on ASB. The study was therefore designed to assess the prevalence of ASB in children with SCA, its determinants, offending pathogens and their antibiogram compared to what obtains in their apparently healthy counterparts seen at the Jos University Teaching Hospital (JUTH). The descriptive cross-sectional and comparative study involving 136 cases and same number of controls aged 2-17 years was carried between January-May 2018.Urinalyses, urine microscopy, culture and sensitivity were performed on samples from all participants. Using urine culture as standard, sensitivity, specificity, positive and negative predictive values of nitrite and leucocyte esterase tests were calculated. Prevalence of ASB in cases was 8.1% compared to 1.5% in controls. Prominent organisms were Escherichia coli (45.5%) and Staphylococcus aureus (27.3%) in cases and Proteus spp (50%) and Providencia spp (50%) in controls. Combined dipstick test had specificity and positive predictive value (PPV) of 100.0% and 100.0% respectively. All organisms isolated from the cases and controls were sensitive to ceftriaxone and meropenem while most were resistant to ampicillin, nitrofurantoin and erythromycin. The female gender was significantly associated with the occurrence of ASB [2 = 8.61, p = 0.003]. Combined dipstick test had an edge over nitrite and leukocyte esterase tests used singly in terms of specificity for the diagnosis of ASB. Female sicklers in Jos-North Local Government Area of Plateau State are unduly predisposed to ASB. Introduction of routine urinalysis screening among SCA children in clinics in Jos and its neighbourhood would be worthwhile.