Aim: To study iron status as a marker of reduced risk of cardiovascular disease in regular blood donors. Objectives: 1. 2. 3. 4. To determine the iron status of regular blood donors in Lagos, Nigeria. To determine the lipid profile of regular blood donors in Lagos, Nigeria. To assess the cardiovascular risk of regular blood donors, as determined by their lipid profile. To determine the association if any between iron status and cardiovascular risk in regular blood donors. Study design: This was a cross sectional study conducted on regular blood donors attending the blood donors’ clinic of Lagos University Teaching Hospital. Materials and Methods: Eighty two (82) participants who satisfied the inclusion criteria were enrolled into the study. Of this number, fifty two (52) were regular blood donors (study group) and thirty (30) were non donors (control group). Total cholesterol (TC), triglycerides (TG), low density lipoprotein (LDL), high density lipoproteins (HDL) and small dense low density lipoproteins (sdLDL) were estimated on the serum samples of all the participants. Subjects’ full blood count, red cell indices and some iron indices (serum iron, serum ferritin and serum transferrin receptors) were also estimated. i Results: There was no difference in the mean Hb concentration and PCV between the study and control populations. The regular blood donors had significantly lower mean serum ferritin (41.46+40.33ng/l) compared with the control group (102.46+80.26ng/l). p=0.000. The TC, LDL and sdLDL in the study group were also significantly lower (4.66+0.86mm/l, 2.32+0.73mm/l and 0.55+0.36mm/l respectively) when compared with the control group (5.61+1.26mm/l, 3.06+0.89mm/l and 1.44+0.41mm/l respectively) with p=<0.05 in all cases. Even though the HDL level was lower in the regular donors, the mean LDL/HDL ratio in the study group (2.90+1.28mm/l) was lower compared with the control group (3.22+1.04mm/l), but the difference was not statistically significant. p=0.247. 57% of the control group had LDL/HDL ratio of > 3, whilst only 42% of the study group had a ratio >3. x2=1.57; p=0.21. Regular blood donors had significantly lower mean sdLDL levels (0.55+0.36mm/l) compared with the controls (1.44+0.41mm/l) p=0.000. Whereas only 23% of the non donor population had sdLDL level less than the cut off value of 0.9065mm/l, 83% of the regular donors had values below 0.9065mm/l. x2=17.86; p=0.000. . Conclusion: Regular blood donation appears to be protective against cardiovascular disease through its lowering of body iron stores and decreased markers of cardiovascular disease