Healthy newborns have been shown to have transient, asymptomatic and self-limiting low blood glucose levels as part of their adaptation to extra-uterine life. Despite this knowledge, the practice of prelacteal feeding by mothers and health care workers as a result of the erroneous perception of hypoglycaemia in healthy newborns that are considered not to have breastfed optimally in the first 24 hours of life has persisted. Prelacteal feeding interferes with the initiation and sustenance of exclusive breastfeeding, with loss of its protective effects, such as prevention of pneumonia and diarrhoeal diseases, amongst others. Thus, this study sought to proffer a profile of blood glucose levels for newborns in the first 24 hours of life, to document clinical signs among hypoglycaemic newborns, as well as to determine the relationship between mode of delivery and blood glucose levels of newborns. The findings may potentially serve as a scientific and educational tool for appropriate counseling. This was a longitudinal study conducted over six weeks among 240 exclusively breastfed healthy term newborns delivered at Obio Cottage Hospital, a secondary health care centre in Rivers State. The Fine test Auto- codingTM glucometer was used to measure blood glucose levels by the bedside at birth, with follow up measurements at 3, 12 and 24 hours of life. Information on mothers’ characteristics such as age, level of education, occupation, marital status and parity, were obtained directly from mothers and from mothers’ case notes.